Bruker micrOTOF II

ESI

TOF

< 2 ppm

> 16,500

Small molecule exact mass measurements

Bruker micrOTOF-Q II

ESI

Q-TOF

1 – 2 ppm

> 17,500

Coupled to ultra-high pressure LC for analysis of complex biological samples

Bruker Autoflex III

MALDI

TOF/TOF

Reflector
<20ppm

Linear
< 50 ppm

Reflector
>20,000

Linear
> 5000

Small and large molecules; PMF; peptide MS/MS; biomolecules ≥ 150 kD

AB Sciex 5500 QTrap

ESI

Q-TRAP

1 ppm

N/A

Multiple Reaction Monitoring (MRM) for quantitation; couple to nano-LC for complex sample analysis

Bruker maXis
(Hummon Group)

ESI

qQ-TOF

1 ppm

> 70,000

Coupled to nano-ultra-high pressure LC for complex proteomic analysis

Thermo Velos Orbitrap 
(Dovichi Group)

API

Linear trap-Orbital trap

< 1 ppm

> 100,000

Complex proteomics and labeling experiments; parallel MS and MSⁿ analyses

Waters GCT Premier
(Innovation Park)

EI, CI, FI

Orthogonal acceleration TOF

< 5 ppm

> 7000

Complex volatile mixture analysis

Waters TQD Acquity Triple Quadrupole
(Innovation Park)

ESI

Triple Quadrupole

1 ppm

Unit Res.

Low level quantitation of biomolecules; metabolomics

INSTRUMENT

SPECIFICATIONS

GE Microcal Isothermal Titration Calorimeter ITC200
(Department of Chemistry and Biochemistry)

• Directly measure sub-millimolar to nanomolar binding constants
• Requires as little as 10 ug of protein
• Available temperatures of 2˚C to 80 ˚C

Jasco Circular Dichroism Spectrometer
(Department of Chemistry and Biochemistry)

• Operational in step-scan or continuous scan mode
• Stopped-flow accessory available
• Applications include protein folding studies and enzyme kinetics

Waters Alliance HPLC
(Innovation Park)

• Coupled to a fraction collector